In suspected sepsis, which action should be performed prior to antibiotic administration?

• A. Administer broad-spectrum antibiotics
• B. Obtain lactate
• C. Obtain cultures prior to antibiotics
• D. Initiate source control

Answer: (C)

Obtaining cultures before giving antibiotics maximizes the chance to identify the causative organism and its susceptibilities, which guides targeted therapy and supports antimicrobial stewardship. Drawing blood cultures (from two sites) and collecting cultures from the suspected source, when feasible, helps ensure results aren’t masked by prior antibiotics and allows de-escalation or adjustment of therapy once results return. While starting antibiotics promptly is critical in suspected sepsis, acquiring cultures first, if it won’t cause delay, provides essential diagnostic information that improves later treatment decisions.